Journal: Journal of Lipid Research

Article Title: A decreased expression of angiopoietin-like 3 is protective against atherosclerosis in apoE-deficient mice

doi: 10.1194/jlr.m300031-jlr200

Figure Lengend Snippet: Fig. 1. Angiopoietin-like 3 (Angptl3) expression in the liver of mice with a recessive mutation in Angptl3 gene causing hypolipidemia (hypl) in the presence (A) and absence (B) of apolipoprotein E (apoE). TaqMan analyses were performed in the liver of 24-week-old males (n 4) fasted for 16 h. The amount of Angptl3 mRNA was corrected by dividing it with that of 36B4 mRNA in each sample. Values are depicted as mean SD. *P 0.05, **P 0.01.

Article Snippet: Total RNA was extracted from the liver using Trizol Reagent (Invitrogen) and subjected to reverse transcription and amplification according to the protocol supplied with the TaqMan Gold RTPCR kit (Perkin-Elmer) for Angptl3 and 36B4 gene.

Techniques: Expressing, Mutagenesis

Journal: Nutrition & Metabolism

Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

doi: 10.1186/s12986-023-00738-z

Figure Lengend Snippet: Hepatic mRNA and protein expression of Pcsk9 and Anxa2 genes after 20 weeks on HFD. The hepatic expression was assessed at mRNA and protein levels by qPCR/ELISA for PCSK9 ( A, B ) and qPCR/Western blot for ANXA2 ( C, D ). The mRNA level was calculated with 2 − ΔΔ Ct method. The relative protein level on the blots was determined by ImageJ, using actin as an internal control. Values are reported as mean ± SD; n = 5 in each group. The p value was determined by unpaired T-test. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: PCSK9 secretion was measured from the conditioned medium using a LEGEND MAXTM Human PCSK9 ELISA Kit (BioLegend, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: Nutrition & Metabolism

Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

doi: 10.1186/s12986-023-00738-z

Figure Lengend Snippet: Lipid accumulation in the livers of PCSK9 −/− mice on control diet. Lipid composition of the liver was examined by HPLC–MS/MS. The concentration of TG, DG and CER subtypes ( A, F, K ), their total amount ( B, G, L ), the proportion of saturated and monounsaturated TGs, DGs and CERs to total amount ( C, D, H, I, M, N ) and the ratio of monounsaturated and saturated lipids were determined ( E, J, O ). Values are reported as mean ± SD; n = 5 in each group. The p value was determined by multiple T-test ( A, F, K ) or unpaired T-test (all others). * p < 0.05

Article Snippet: PCSK9 secretion was measured from the conditioned medium using a LEGEND MAXTM Human PCSK9 ELISA Kit (BioLegend, USA).

Techniques: Control, Tandem Mass Spectroscopy, Concentration Assay

Journal: Nutrition & Metabolism

Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

doi: 10.1186/s12986-023-00738-z

Figure Lengend Snippet: Hepatic gene expression of Pcsk9 and Anxa2 genes in PCSK9 −/− mice on control diet. The hepatic expression was assessed at mRNA and protein levels by qPCR/ELISA for PCSK9 ( A, B ) and qPCR/Western blot for (ANXA2; C, D ). The mRNA level was calculated with 2 − ΔΔ Ct method. The relative protein level on the blots was determined by ImageJ, using actin as an internal control. Values are reported as mean ± SD; n = 5 in each group. The p value was determined by multiple T-test ( A and B ). * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: PCSK9 secretion was measured from the conditioned medium using a LEGEND MAXTM Human PCSK9 ELISA Kit (BioLegend, USA).

Techniques: Gene Expression, Control, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Nutrition & Metabolism

Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

doi: 10.1186/s12986-023-00738-z

Figure Lengend Snippet: Hepatic gene expression of Cd36 and Ldlr genes in PCSK9 −/− mice. Hepatic expression at mRNA level was measured by qPCR and calculated with 2 − ΔΔ Ct method ( A, C ). The relative protein level on the blots was determined by ImageJ, using actin as an internal control ( B, D ). Values are reported as mean ± SD; n = 5 in each group. The p value was determined by multiple T-test ( A and B ). * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: PCSK9 secretion was measured from the conditioned medium using a LEGEND MAXTM Human PCSK9 ELISA Kit (BioLegend, USA).

Techniques: Gene Expression, Expressing, Control

Journal: Nutrition & Metabolism

Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

doi: 10.1186/s12986-023-00738-z

Figure Lengend Snippet: Co-localization of PCSK9 with LDLR, CD36 and ANXA2. HepG2 cells were examined for PCSK9 secretion 16 and 40 h after serum depletion ( A , n = 3). Cellular localization of PCSK9, LDLR, CD36 and ANXA2 proteins was examined by laser scanning confocal microscopy ( B ). Co-localization rate was used to quantify the degree of co-localization ( C , n = 5). The scale bar has a width of 5 µm. Values are reported as mean ± SD. The p value was determined by unpaired T-test ( A ) or one-way ANOVA combined with Tukey’s multiple comparisons test ( C ). * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: PCSK9 secretion was measured from the conditioned medium using a LEGEND MAXTM Human PCSK9 ELISA Kit (BioLegend, USA).

Techniques: Serum Depletion, Confocal Microscopy

Journal: Nutrition & Metabolism

Article Title: High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes

doi: 10.1186/s12986-023-00738-z

Figure Lengend Snippet: Overview figure summarizing the content of the manuscript. The figure represents changes in PCSK9, LDLR, ANXA2 and CD36 levels after 20 weeks HFD and in PCSK9 gene knockout mice. The quantity of the colour coded proteins is proportional with the amount found under experimental conditions. Changes in different lipid levels are listed in the tables

Article Snippet: PCSK9 secretion was measured from the conditioned medium using a LEGEND MAXTM Human PCSK9 ELISA Kit (BioLegend, USA).

Techniques: Gene Knockout

Journal: Arthritis Research & Therapy

Article Title: Semaphorin 3G exacerbates joint inflammation through the accumulation and proliferation of macrophages in the synovium

doi: 10.1186/s13075-022-02817-7

Figure Lengend Snippet: Attenuated joint inflammation in Sema3G-deficient mice. A The clinical score of Sema3G-deficient (Sema3G −/− ) mice and their littermate controls (Sema3G +/− ) in CIA. Data were obtained from four independent experiments ( N = 17 in each group). B The pathological scores of CIA. The front paws were subjected to H&E staining, and the inflammation, the cartilage damage, and the erosion scores were separately assessed. C The inflammatory cytokines in sera. The sera were collected on day 42 and subjected to ELISA to measure IL-6 and TNFα. D The clinical score of Sema3G −/− mice and Sema3G +/− in CAIA. Data were obtained from four independent experiments ( N = 13 in each group). E The pathological scores of CAIA. The front paws were subjected to H&E staining, and the inflammation, the cartilage damage, and the erosion scores were separately assessed. F The inflammatory cytokines in sera. The sera were collected on day 9 and subjected to ELISA to measure IL-6 and TNFα. Data are expressed as the means ± SEM. For the analyses of the clinical score, 2-way ANOVA was used. For the pathological scores, an unpaired t -test was used

Article Snippet: The serum levels of IL-6 and TNFα were measured using a mouse ELISA MAX Standard IL-6 kit (BioLegend) and a mouse ELISA MAX Standard TNFα kit (BioLegend).

Techniques: Staining, Enzyme-linked Immunosorbent Assay

Journal: Cell Reports Medicine

Article Title: Soluble trivalent engagers redirect cytolytic T cell activity toward tumor endothelial marker 1

doi: 10.1016/j.xcrm.2021.100362

Figure Lengend Snippet:

Article Snippet: Human IL-2 ELISA MAX Standard kit , Biolegend , CAT#431801.

Techniques: Recombinant, Apoptosis Assay, Cell Isolation, Lactate Dehydrogenase Assay, Enzyme-linked Immunosorbent Assay, Software, Transfection, Luciferase